Nuclear ribosomal RNA genes of B. komarovianum, B. scorzoneraefolium B. longiradiatum, and B. euphorbioides have been analyzed by means of Southern blot hybridization and the sequencing of internal transcribed spacer regions to figure out the molecualr variation of the genus. Five restriction enzymes including EcoRI and BamHI were used to digest genomic DNAs. EcoRI digestion produced the repeating units of 1.8 Kb and 3.6 Kb restriction fragments, and BamHI digestion produced 1.2 Kb and 2.6 Kb repeating fragments. We have confirmed the restriction site variation and the length variation of ribisomal DNA in Bupleurum. Three trees were constructed on the base of restriction fragment length polymorphism and the sequences of ITS region; B. komarovianum and B. scorzoneraefolium were clustered as one sister group and B. longiradiatum and B. euphorbioides as another one.
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