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Korean J. Pl. Taxon > Volume 48(3); 2018 > Article
Korean Journal of Plant Taxonomy 2018;48(3): 195-200.
doi: https://doi.org/10.11110/kjpt.2018.48.3.195
Isolation and characterization of EST-SSR markers for Astilboides tabularis (Saxifragaceae), endangered species in Korea
Eui-Kwon JUNG1 , Dae-Hyun KANG2 , Ki-Oug YOO3 , Myounghai KWAK4 , Young-Dong KIM1 , Bo-Yun KIM2
1Department of Life Science, Hallym University, Chuncheon 24252, Korea
2Multidisciplinary Genome Institute, Hallym University, Chuncheon 24252, Korea
3Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Korea
4Plant Resources Division, National Institute of Biological Resources, Incheon 22689, Korea
Correspondence :  Bo-Yun KIM ,Email: wken416@hallym.ac.kr
Received: September 4, 2017  Revised: September 17, 2018   Accepted: September 28, 2018
Abstract
Genetic assessments of rare and endangered species are among the first steps necessary to establish the proper management of natural populations. Transcriptome-derived single-sequence repeat markers were developed for the Korean endangered species Astilboides tabularis (Saxifragaceae) to assess its genetic diversity. A total of 96 candidate microsatellite loci were isolated based on transcriptome data using Illumina pair end sequencing. Of these, 26 were polymorphic, with one to five alleles per locus in 60 individuals from three populations of A. tabularis. The observed and expected heterozygosity per locus ranged from 0.000 to 0.950 and from 0.000 to 0.741, respectively. These polymorphic transcriptome-derived simple sequence repeat markers would be invaluable for future studies of population genetics and for ecological conservation of the endangered species A. tabularis.
KeyWords: Astilboides tabularis, endangered species, EST-SSR markers, next-generation sequencing, conservation
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