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Korean J. Pl. Taxon > Volume 48(3); 2018 > Article
Korean Journal of Plant Taxonomy 2018;48(3): 206-217.
doi: https://doi.org/10.11110/kjpt.2018.48.3.206
Phylogenetic analysis of Viburnum (Adoxaceae) in Korea using DNA sequences
Yun Gyeong CHOI1, Jung Won YOUM1, Chae Eun LIM2 , Sang-Hun OH1
1Department of Biology, Daejeon University, Daejeon 34520, Korea
2Plant Resources Division, National Institute of Biological Resources, Incheon 22689, Korea
Correspondence :  Sang-Hun OH ,Email: soh42@dju.kr
Received: September 1, 2018  Revised: September 20, 2018   Accepted: September 29, 2018
Abstract
The nucleotide sequences of the chloroplast rbcL, matK, and psbA-trnH and nuclear internal transcribed spacer (ITS) regions were determined from all species of Viburnum in Korea with multiple accessions to reconstruct the phylogeny and to evaluate the utility of the DNA sequences as DNA barcodes. The results of phylogenetic analyses of the cpDNA and ITS data are consistent with the findings of previous studies of Viburnum. Four morphologically closely related species, V. dilatatum, V. erosum, V. japonicum, and V. wrightii, were included in a strongly supported sister clade of V. koreanum and V. opulus. Viburnum odoratissimum is suggested to be sister to the V. dilatatum/V. koreanum clade in the cpDNA data, while V. odoratissimum is a sister to V. furcatum in the ITS data. Viburnum burejaeticum and V. carlesii are strongly supported as monophyletic. Our analyses of DNA barcode regions from multiple accessions of the species of Viburnum in Korea confirm that six out of ten species in Korea can be discriminated at the species level. The V. dilatatum complex can be separated from the remaining species according to molecular data, but the resolution power to differentiate a species within the complex is weak. This study suggests that regional DNA barcodes are useful for molecular species identification in the case of Viburnum when flowering or fruiting materials are not available.
KeyWords: DNA barcode, morphology, relationship, species identification, Viburnum
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