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Korean J. Pl. Taxon > Volume 41(4); 2011 > Article
Korean Journal of Plant Taxonomy 2011;41(4): 299-314.
doi: https://doi.org/10.11110/kjpt.2011.41.4.299
총설 : Microsatellite 개발 및 분석법에 대한 소개
윤영은 , 유정남 , 이병윤 , 곽명해
An Introduction to Microsatellite Development and Analysis
Young Eun Yun, Jeong Nam Yu, Byoung Yoon Lee, Myoung Hai Kwak
Received: October 3, 2011  Revised: November 4, 2011   Accepted: December 14, 2011
The choice of molecular markers is the first step when selecting experimental plans in the field of population genetics. The popular molecular markers in population genetic studies are mainly allozyme, RAPD, RFLP, AFLP, microsatellite, SNP and ISSR. Among these, microsatellites are frequently found in nuclear, chloroplast and mitochondrial genome, showing a high level of polymorphism and nuclear microsatellites are codominant. Thus, it is a favorable molecular marker for population structure analyses and genetic diversity studies. Microsatellites are composed of tandem repeated 1~6 base pair nucleotide motifs and can be easily amplified by PCR reactions using locus specific primers. Because microsatellites have low cross-species transferability, however, they are only applicable between phylogenetically close species. In wild plants, the lack of genomic information and the high development cost of the microsatellite obstruct the wider use of microsatellites in plant population genetics research. In this review, we introduce the basis for microsatellite markers, the development process, and analytical methods as well as evolutionary models and their applications. In addition, possible genotyping errors which lead to erroneous conclusions are discussed.
KeyWords: Microsatellite, Genotyping, Population Genetics, Molecular Marker
주요어: 유전자형, 집단유전학, 분자마커
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