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Korean J. Pl. Taxon > Volume 41(4); 2011 > Article
Korean Journal of Plant Taxonomy 2011;41(4): 324-328.
doi: https://doi.org/10.11110/kjpt.2011.41.4.324
한국산 흰명아주와 근연종의 세포분류학적 연구
정영재 , 김무열 , 이병순
Cytotaxonomical Study of the Chenopodium album and its Related Species in Korea
Young Jae Chung, Mu Yeol Kim, Byong Soon Lee
Received: October 27, 2011  Revised: December 6, 2000   Accepted: December 13, 2000
The purpose of this study was to analyze the interspecific relationships of Chenopodium album and its related taxa collected in Korea. The 18S-26S ribosomal DNA (45S rDNA) loci were detected directly on mitotic chromosomes by fluorescence in situ hybridization (FISH) and the chromosome numbers were examined using aceto-orcein methods. The chromosomal numbers of Chenopodium album var. album and C. album var. centrorubrum were 2n = 6x = 54, whereas for C. album var. stenophyllum, this number was 2n = 4x = 36. The basic chromosome number was x = 9. The biotin labeled 18S-26S rDNA probe exhibited eight yellow fluorescent signals on the metaphase chromosome of C. album var. album and var. centrorubrum respectively, while two yellow signals of C. album var. stenophyllum were noted. All of the signals on the chromosomes were located at the terminal regions. The chromosome number and FISH findings suggest that C. album var. centrorubrum is merged into var. album and that it is clearly distinguished from C. album var. stenophyllum.
KeyWords: Chenopodium album, chromosome, 45S rDNA, FISH
주요어: 흰명아주, 염색체
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