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Korean J. Pl. Taxon > Volume 43(2); 2013 > Article
Korean Journal of Plant Taxonomy 2013;43(2): 139-145.
doi: https://doi.org/10.11110/kjpt.2013.43.2.139
정선황기의 세포유전학적 연구
한상은 , 김현희 , 허권
A cytogenetic study of Astragalus koraiensis Y.N.Lee
Sang Eun Han, Hyun Hee Kim, Kweon Heo
Abstract
This study was carried out to determine the kayotype and chromosomal localizations of 45S and 5S rDNAs using FISH in Astragalus koraiensis. The somatic metaphase chromosome number of this species was 2n=16 with basic chromosome number of x=8. The karyotype of A. koraiensis was consisted of six pairs of median region chromosomes (chromosome 1,3,4,5,6,8) and two pairs of submedian chromosomes(chromosome 2,7). Based on the FISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 5. Whereas, two pair of 5S site were detected on the short arm of chromosome 4 and centromeric region of chromosome 7, respectively. These are quite different patterns from A. membranaceus, A. menbranaceus var. alpinus, and A. mongholicus. Although A. koraiensis is considered as Korean endemic species, therefore, it should be conducted out comparative FISH study with A. sikokianus and A. bhotanensis which are very similar to A. koraiensis morphologically.
KeyWords: FISH, Rdna, Astragalus L, Astragalus koraiensis, karyotype analysis, FISH, Rdna
주요어: 황기속, 정선황기, 핵형 분석
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