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Korean J. Pl. Taxon > Volume 41(2); 2011 > Article
Korean Journal of Plant Taxonomy 2011;41(2): 119-129.
doi: https://doi.org/10.11110/kjpt.2011.41.2.119
PCR-RFLP와 ITS 염기서열 분석을 이용한 한국산 초롱꽃과 (Campanulaceae)의 계통유연관계
김경아 , 유기억
Phylogenetic Relationships of Korean Campanulaceae Based on PCR-RFLP and ITS Sequences
Kyung Ah Kim, Ki Oug Yoo
Received: May 26, 2011   Accepted: June 7, 2011
Abstract
Phylogenetic studies were conducted to evaluate the taxonomic relationships among 27 taxa, including 2 outgroups of Korean Campanulaceae, using PCR-RFLP analysis and ITS sequences. In the PCR-RFLP analysis, 15 restriction endonucleases produced 244 restriction sites and size variations from the chloroplast DNA, and 59 restriction sites (24%) showed polymorphism. The length of the ITS regions ranged from 588 bp to 797 bp. The sequence divergence including the outgroups is 0-39.36%. Phylogenetic analyses based on PCR-RFLP and ITS data suggest that Campanulaceae is monophyletic; Codonopsis and Platycodon forms an independent clade; the Peracarpa and Asyneuma clade is a sister to the Adenophora-Hanabusaya clade; Campanula is monophyletic; and Wahlenbergia basally branches within the ITS tree, whereas they are placed between Campanula and the Codonopsis-Platycodon clade in the PCR-RFLP tree; Hanabusaya is placed within the Adenophora clade; and Adenophora is paraphyletic and shows discordance to the infrageneric classifications based on morphological data. The present results show two data sets, largely congruent at the generic level, but their phylogenetic positions, in particular the Wahlenbergia and Hanabusaya and the infrageneric classifications in Adenophora, show some incongruence.
KeyWords: Campanulaceae, phylogenetic relationships, PCR-RFLP, ITS
주요어: 초롱꽃과, 계통유연관계
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